Anti-Phospho-MARK2 T595 Antibody

Product Name

Anti-Phospho-MARK2 T595 Antibody

View all MARK2 Antibodies

SKU/Catalog Number

P02117

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Phospho-MARK2 T595 Antibody (Catalog # P02117). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Phospho-MARK2 T595 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02117)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding T595 of Human MARK2 isoform a.  The immunogen peptide was phosphorylated at the residue corresponding to T595.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P02117 is reactive to MARK2 in Human

Applications

P02117 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

87.911kDa

Background of MARK2

MARK2 pT595 antibody is suitable for Cancer, Immunology and Nuclear Signaling research. This antibody recognizes a regulatory phosphorylation on a kinase that is critical to regulation of microtubules, cell cycle and immune responses. MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer.  Several cDNA clones have been isolated that encode two isoforms of the human ser/thr protein kinase EMK1 called MARK2.  These isoforms were characterized by the presence of a 162-bp alternative exon that gives rise to two forms, one containing the exon and the other one lacking it.  Both forms were found to be co-expressed in a number of selected cell lines and tissue samples.  Human MARK2 was shown to be encoded by a single mRNA that is ubiquitously expressed. This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein. Multiple splice variants exist for this protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:1,000 - 1:3,000
IF Microscopy: User optimized
WB: 1:250 - 1:1,000

Validation Images & Assay Conditions

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Western blot analysis of MARK2 expression in wild type Jurkat cells (lane 1) and wild type Jurkat cells stimulated with CD3/CD28 (T-cell receptor stimulation) (lane 2). MARK2 at 83KD was detected using rabbit anti-MARK2 pT595 Antigen Affinity purified polyclonal antibody (Catalog # P02117) at 1:500.

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Western blot analysis of MARK2 expression in 293T cells untransfected (lane 1), 293T cells transfected with MARK2 and PKC-zeta (lane 2) and 293T cells transfected with MARK2 (lane 3). MARK2 at 83KD was detected using rabbit anti-MARK2 pT595 Antigen Affinity purified polyclonal antibody (Catalog # P02117) at 1:1000.

Gene/Protein Information For MARK2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.11; EC 2.7.11.1; ELKL motif kinase 1; ELKL motif kinase; EMK-1; EMK1PAR-1; MAP/microtubule affinity-regulating kinase 2MGC99619; PAR1 homolog; Par1b; Ser/Thr protein kinase PAR-1B; serine/threonine protein kinase EMK; serine/threonine-protein kinase MARK2 MARK2 EMK-1, EMK1, PAR-1, Par-1b, Par1b microtubule affinity regulating kinase 2 serine/threonine-protein kinase MARK2|ELKL motif kinase 1|MAP/microtubule affinity-regulating kinase 2|PAR1 homolog b|Ser/Thr protein kinase PAR-1B|serine/threonine protein kinase EMK|testicular tissue protein Li 117

For more info on MARK2, check out the MARK2 Infographic

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In this infographic, you will see the following information for MARK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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